Hayato Ota | Faculty List | Soka University

Assistant Lecturer

Hayato Ota

Profile

Faculty of Science and Engineering Department of Life Science and Technology

Faculty of Science and Engineering Department of Science and Engineering for Sustainable Innovation

Specialized Field	Glycobiology, Cell Biology
Research theme	Functional analysis of glycans in pluripotent stem cells Functional analysis of glycans in prostate cancer
research content	Glycans are called the "third chain of life" after proteins and nucleic acids, which play important roles in living organisms. Many of the proteins and lipids present on the cell surface have glycans, and these glycans are deeply involved in communication between cells and intracellular signal transduction, controlling vital phenomena. My research involves analyzing the function of glycans in mouse and human pluripotent stem cells (iPS cells and ES cells). Glycans play an important role in maintaining the undifferentiated state of pluripotent stem cells and controlling their differentiation, and elucidating these mechanisms will not only deepen our understanding of molecular biology, but will also lead to elucidating the developmental mechanisms of mammals and improving culture techniques for regenerative medicine. My research also focuses on prostate cancer. The emergence of castration-resistant prostate cancer, which is resistant to drugs and castration therapy, is considered a problem during the course of prostate cancer treatment. Currently, there is no established treatment for castration-resistant prostate cancer, but in my research to date, I have revealed that the acquisition of resistance in prostate cancer is caused by increased expression of 3-sulfated heparan sulfate. Currently, I am focusing on other glycans as well, with the aim of elucidating the mechanism of resistance acquisition and contributing to the development of new treatments.
Subjects in charge	Basic science experiments, biology experiments, chemistry experiments
Main career, work history, and academic background	2016 Graduated from the Department of Bioinformatics and Engineering, Faculty of Engineering, Soka University 2018: Completed Master's degree in Bioinformatics Engineering at the Graduate School of Engineering, Soka University 2023: Graduated from the Graduate School of Engineering at Soka University, Department of Bioinformatics and Engineering (PhD in Engineering)
Affiliated academic societies and organizations	Japan Society for Regenerative Medicine International Society for Stem Cell Research (ISSCR) Molecular Biology Society of Japan Japanese Biochemical Society Japanese Society of Carbohydrate Research Society for Glycobiology
Main Papers and Publications	Ota, H., Sato, H., Mizumoto, S., Wakai, K., Yoneda, K., Yamamoto, K., Nakanishi, H., Ikeda, J. I., Sakamoto, S., Ichikawa, T., Yamada, S., Takahashi, S., Ikehara, Y., & Nishihara, S. (2023). Switching mechanism from AR to EGFR signaling via 3-O-sulfated heparan sulfate in castration-resistant prostate cancer. Scientific reports, 13(1), 11618. Murakami, K., Tamura, R., Ikehara, S., Ota, H., Ichimiya, T., Matsumoto, N., Matsubara, H., Nishihara, S., Ikehara, Y., & Yamamoto, K. (2023). Construction of mouse cochlin mutants with different GAG-binding specificities and their use for immunohistochemistry. The Biochemical journal, 480(1), 41–56. Ota, H., & Nishihara, S. (2022). Regulation of 3-O-Sulfation of Heparan Sulfate During Transition from the Naïve to the Primed State in Mouse Embryonic Stem Cells. Methods in molecular biology (Clifton, N.J.), 2303, 443–452. Pecori, F., Yokota, I., Hanamatsu, H., Miura, T., Ogura, C., Ota, H., Furukawa, J. I., Oki, S., Yamamoto, K., Yoshie, O., & Nishihara, S. (2021). A defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition. Scientific reports, 11(1), 1276. Miura, T., Yuasa, N., Ota, H., Habu, M., Kawano, M., Nakayama, F., & Nishihara, S. (2019). Highly sulfated hyaluronic acid maintains human induced pluripotent stem cells under feeder-free and bFGF-free conditions. Biochemical and biophysical research communications, 518(3), 506–512. Shinchi, H., Nakamura, T., Ota, H., Nishihara, S., Wakao, M., & Suda, Y. (2019). Cell Profiling Based on Sugar-Chain-Cell Binding Interaction and Its Application to Typing and Quality Verification of Cells. Chembiochem : a European journal of chemical biology, 20(14), 1810–1816. Miura, T., Ota, H., & Nishihara, S. (2017) Atmospheric Pressure Plasma Irradiation on Embryonic Stem Cells: Signals and Differentiation. Plasma Medicine, 7(3), 215-225.

Specialized Field	Glycobiology, Cell Biology
Research theme	Functional analysis of glycans in pluripotent stem cells Functional analysis of glycans in prostate cancer
research content	Glycans are called the "third chain of life" after proteins and nucleic acids, which play important roles in living organisms. Many of the proteins and lipids present on the cell surface have glycans, and these glycans are deeply involved in communication between cells and intracellular signal transduction, controlling vital phenomena. My research involves analyzing the function of glycans in mouse and human pluripotent stem cells (iPS cells and ES cells). Glycans play an important role in maintaining the undifferentiated state of pluripotent stem cells and controlling their differentiation, and elucidating these mechanisms will not only deepen our understanding of molecular biology, but will also lead to elucidating the developmental mechanisms of mammals and improving culture techniques for regenerative medicine. My research also focuses on prostate cancer. The emergence of castration-resistant prostate cancer, which is resistant to drugs and castration therapy, is considered a problem during the course of prostate cancer treatment. Currently, there is no established treatment for castration-resistant prostate cancer, but in my research to date, I have revealed that the acquisition of resistance in prostate cancer is caused by increased expression of 3-sulfated heparan sulfate. Currently, I am focusing on other glycans as well, with the aim of elucidating the mechanism of resistance acquisition and contributing to the development of new treatments.
Subjects in charge	Basic science experiments, biology experiments, chemistry experiments
Main career, work history, and academic background	2016 Graduated from the Department of Bioinformatics and Engineering, Faculty of Engineering, Soka University 2018: Completed Master's degree in Bioinformatics Engineering at the Graduate School of Engineering, Soka University 2023: Graduated from the Graduate School of Engineering at Soka University, Department of Bioinformatics and Engineering (PhD in Engineering)
Affiliated academic societies and organizations	Japan Society for Regenerative Medicine International Society for Stem Cell Research (ISSCR) Molecular Biology Society of Japan Japanese Biochemical Society Japanese Society of Carbohydrate Research Society for Glycobiology
Main Papers and Publications	Ota, H., Sato, H., Mizumoto, S., Wakai, K., Yoneda, K., Yamamoto, K., Nakanishi, H., Ikeda, J. I., Sakamoto, S., Ichikawa, T., Yamada, S., Takahashi, S., Ikehara, Y., & Nishihara, S. (2023). Switching mechanism from AR to EGFR signaling via 3-O-sulfated heparan sulfate in castration-resistant prostate cancer. Scientific reports, 13(1), 11618. Murakami, K., Tamura, R., Ikehara, S., Ota, H., Ichimiya, T., Matsumoto, N., Matsubara, H., Nishihara, S., Ikehara, Y., & Yamamoto, K. (2023). Construction of mouse cochlin mutants with different GAG-binding specificities and their use for immunohistochemistry. The Biochemical journal, 480(1), 41–56. Ota, H., & Nishihara, S. (2022). Regulation of 3-O-Sulfation of Heparan Sulfate During Transition from the Naïve to the Primed State in Mouse Embryonic Stem Cells. Methods in molecular biology (Clifton, N.J.), 2303, 443–452. Pecori, F., Yokota, I., Hanamatsu, H., Miura, T., Ogura, C., Ota, H., Furukawa, J. I., Oki, S., Yamamoto, K., Yoshie, O., & Nishihara, S. (2021). A defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition. Scientific reports, 11(1), 1276. Miura, T., Yuasa, N., Ota, H., Habu, M., Kawano, M., Nakayama, F., & Nishihara, S. (2019). Highly sulfated hyaluronic acid maintains human induced pluripotent stem cells under feeder-free and bFGF-free conditions. Biochemical and biophysical research communications, 518(3), 506–512. Shinchi, H., Nakamura, T., Ota, H., Nishihara, S., Wakao, M., & Suda, Y. (2019). Cell Profiling Based on Sugar-Chain-Cell Binding Interaction and Its Application to Typing and Quality Verification of Cells. Chembiochem : a European journal of chemical biology, 20(14), 1810–1816. Miura, T., Ota, H., & Nishihara, S. (2017) Atmospheric Pressure Plasma Irradiation on Embryonic Stem Cells: Signals and Differentiation. Plasma Medicine, 7(3), 215-225.

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