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Specially Appointed Professor/URA

Kiyohiko Angata

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Glycan & Life System Integration Center
Specialized Field

Glycobiology, Molecular biology, Biochemistry, Developmental neurobiology
Research theme

Expression and functional regulation of glycosyltransferases
The role of glycans in development
Application of glycan analysis technology
research content

We have been analyzing the role of glycans in the development of the brain and nerves and the malignant progression of cancer, focusing on glycosyltransferases, using molecular biology, biochemistry, and cell biology techniques, RNA-Seq, knockout mice, and glycan DBs. We are also developing technologies (measurement techniques, imaging, and targeting) to analyze new functions of glycans (genes) and apply biomarkers to medicine and industry.
In addition, as a URA, I will promote and manage collaborative research between Soka University researchers and research institutes at other universities and other institutions, and will also engage in outreach activities in glycoscience research.
Main career, work history, and academic background

1995: Doctor of Science, Department of Biophysical Chemistry, Graduate School of Biological Sciences, University of Tsukuba

After working at the La Jolla Cancer Research Foundation (now the Sanford Burnham Prebys Medical Discovery Institute),

2011 Visiting Researcher, National Institute of Advanced Industrial Science and Technology

2021 Researcher, Soka University Glycan & Life System Integration Center

2023 - Present Specially Appointed Professor, Soka University Glycan & Life System Integration Center
Affiliated academic societies and organizations

Japan Molecular Biology Society, Society for Glycobiology, Asian Community of Glycoscience and Glycotechnology (ACGG)
Main Papers and Publications

Original Papers (2000 and after)

  1. Murata A, Angata K, Sogabe M, Sato S, Ichida T, Narimatsu H, Genda T. Serum O-glycosylated hepatitis B surface antigen levels in patients with chronic hepatitis B during nucleos(t)ide analog therapy. BMC Gastroenterol. 2022. 22(1):270.
  2. Angata K, Wagatsuma T, Togayachi A, Sato T, Sogabe M, Tajiri K, Ozawa T, Nagashima I, Shimizu H, Iijima S, Korenaga M, Kuno A, Kaji H, Mizokami M, Narimatsu H. O-Glycosylated HBsAg peptide can induce specific antibody neutralizing HBV infection. Biochim. Biophys. Acta Gen. Subj. 2022 1866(1):130020.
  3. Boottanun P, Ino Y, Shimada K, Hiraoka N, Angata K, Narimatsu H. Correlation of the expression of core 3 synthase with survival outcomes of cholangiocarcinoma patients. Oncol. Lett. 2021. 22(5):760.
  4. Nagai-Okatani C, Zou X, Fujita N, Sogabe I, Arakawa K, Nagai M, Angata K, Zhang Y, Aoki-Kinoshita KF, Kuno A. LM-GlycomeAtlas Ver. 2.0: An integrated visualization for lectin microarray-based mouse tissue glycome mapping data with lectin histochemistry. J. Proteome. Res. 2021. 20(4):2069-2075.　
  5. Doi N, Ino Y, Angata K, Shimada K, Narimatsu H, Hiraoka N. Clinicopathological significance of core 3 O-glycan synthetic enzyme, β1,3-N-acetylglucosaminyltransferase 6 in pancreatic ductal adenocarcinoma. PLoS One. 2020. 15(11):e0242851.
  6. Angata K, Sawaki H, Tsujikawa S, Ocho M, Togayachi A, Narimatsu H. Glycogene expression profiling of hepatic cells by RNA-Seq analysis for glyco-biomarker identification. Front Oncol. 2020. 10:1224.
  7. Ito K, Angata K, Kuno A, Okumura A, Sakamoto K, Inoue R, Morita N, Watashi K, Wakita T, Tanaka Y, Sugiyama M, Mizokami M, Yoneda M, Narimatsu H. Screening siRNAs against host glycosylation pathways to develop novel anti-viral agents against hepatitis B virus. Hepatol. Res. 2020. 50(10):1128-1140.
  8. Silsirivanit A, Matsuda A, Kuno A, Tsuruno C, Uenoyama Y, Seubwai W, Angata K, Teeravirote K, Wongkham C, Araki N, Takahama Y, Wongkham S, Narimatsu H. Multi-serum glycobiomarkers improves the diagnosis and prognostic prediction of cholangiocarcinoma. Clin. Chim. Acta. 2020. 510:142-149.
  9. Yamada I, Shiota M, Shinmachi D, Ono T, Tsuchiya S, Hosoda M, Fujita A, Aoki NP, Watanabe Y, Fujita N, Angata K, Kaji H, Narimatsu H, Okuda S, Aoki-Kinoshita KF. The GlyCosmos Portal integrating glycoscience-related omics data. Nat. Methods. 2020. 17(7):649-650.
  10. Nagai-Okatani C, Aoki-Kinoshita KF, Kakuda S, Nagai M, Hagiwara K, Kiyohara K, Fujita N, Suzuki Y, Sato T, Angata K, Kuno A. LM-GlycomeAtlas Ver. 1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections. Molecules. 2019. 24, 2962.
  11. Nakane T, Angata K, Sato T, Kaji H, Narimatsu H. Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2. J. Biol. Chem. 2019. 294:7433-7444.
  12. Wagatsuma T, Kuno A, Angata K, Tajiri K, Takahashi J, Korenaga M, Mizokami M, Narimatsu H. Highly sensitive glycan profiling of hepatitis B viral particles and a simple method for Dane particle enrichment. Anal. Chem. 2018. 90:10196-10203.
  13. Suzuki-Anekoji M, Suzuki A, Wu SW, Angata K, Murai KK, Sugihara K, Akama TO, Khoo KH, Nakayama J, Fukuda MN, Fukuda M. In vivo regulation of steroid hormones by the Chst10 sulfotransferase in mouse. J. Biol. Chem. 2013. 288: 5007-5016.
  14. Yoneyama T, Angata K, Bao X, Courtneidge S, Chanda SK, Fukuda M. Fer kinase regulates cell migration through a-dystroglycan glycosylation. Mol. Biol. Cell 2012. 23: 771-780.
  15. Suzuki-Anekoji M, Suzuki M, Kobayashi T, Sato Y, Nakayama J, Suzuki A, Bao X, Angata K, Fukuda M. HNK-1 glycan functions as a tumor suppressor for astrocytic tumor. J. Biol. Chem. 2011. 286: 32824-32833.
  16. Bao X, Kobayashi M, Hatakeyama S, Angata K, Gullberg D, Nakayama J, Fukuda MN, Fukuda M. Tumor suppressor function of laminin-binding a-dystroglycan requires a distinct β3-N-acetylglucosaminyltransferase. Proc. Natl. Acad. Sci. U.S.A. 2009. 106:12109-12114.
  17. Miyazaki T, Angata K, Seeberger PH, Hindsgaul O, Fukuda M. CMP substitutions preferentially inhibit polysialic acid synthesis. Glycobiology 2008. 18: 187-194.
  18. Angata K, Huckaby V, Ranscht B, Terskikh A, Marth JD, Fukuda M. Polysialic acid-directed migration and differentiation of neural precursors are essential for mouse brain development. Mol. Cell. Biol. 2007. 27: 6659-6668.
  19. Suzuki M, Suzuki M, Nakayama J, Suzuki A, Angata K, Chen S, Sakai K, Hagihara K, Yamaguchi Y, Fukuda M. Polysialic acid facilitates tumor invasion by glioma cells. Glycobiology 2005. 15: 887-894.
  20. Angata K, Long JM, Bukalo O, Lee W, Dityatev A, Wynshaw-Boris A, Schachner M, Fukuda M, Marth JD. Sialyltransferase ST8Sia-II assembles a subset of polysialic acid that directs hippocampal axonal targeting and promotes fear behavior. J. Biol. Chem. 2004. 279: 32603-32613.
  21. Angata K, Chan D, Thibault J, Fukuda M. Molecular dissection of the ST8Sia IV polysialyltransferase: distinct domains are required for NCAM recognition and polysialylation. J. Biol. Chem. 2004. 279: 25883-25890.
  22. El-Battari A, Prorok M, Angata K, Mathieu S, Zerfaoui M, Ong E, Suzuki M, Lombardo D, Fukuda M. Different glycosyltransferases are differentially processed for secretion, dimerization and autoglycosylation. Glycobiology 2003. 13: 941-953.
  23. Suzuki M, Angata K, Nakayama J, Fukuda M. Polysialic acid and mucin-type O-glycans on the neural cell adhesion molecule (NCAM) differentially regulate myoblast fusion. J. Biol. Chem. 2003. 278: 49459-49468.
  24. Angata K, Suzuki M, Fukuda M. ST8Sia II and ST8Sia IV polysialyltransferases exhibit marked differences in utilizing various acceptors containing oligosialic acid and short polysialic acid: the basis for cooperative polysialylation by two enzymes. J. Biol. Chem. 2002. 277: 36808-36817.
  25. Ong E, Suzuki M, Belot F, Yeh JC, Franceschini I, Angata K, Hindsgaul O, Fukuda M. Biosynthesis of HNK-1 glycans on O-linked oligosaccharides attached to the neural cell adhesion molecule (NCAM): The requirement for core2 β1,6-N-acetylglucosaminyltransferase and the muscle specific domain in NCAM. J. Biol. Chem. 2002. 277: 18182-18190.
  26. Mahal LK, Charter NW, Angata K, Fukuda M, Koshland DE Jr, Bertozzi CR. A small-molecule modulator of poly-a2,8-sialic acid expression on cultured neurons and tumor cells. Science 2001. 294: 380-382.
  27. Suzuki A, Hiraoka N, Suzuki M, Angata K, Misra AK, McAuliffe J, Hindsgaul O, Fukuda M. Molecular cloning and expression of a novel human β-Gal-3-O-sulfotransferase that acts preferentially on N-acetyllactosamine in N- and O-glycans. J. Biol. Chem. 2001. 276: 24388-24395.
  28. Franceschini I, Angata K, Ong E, Hong A, Doherty P, Fukuda M. Polysialyltransferase ST8Sia II (STX) polysialylates all of the major isoforms of NCAM and facilitates neurite outgrowth. Glycobiology 2001. 11: 231-239.
  29. Angata K, Yen TY, El-Battari A, Macher BA, Fukuda M. Unique disulfide bond structures found in ST8Sia IV polysialyltransferase are required for its activity. J. Biol. Chem. 2001. 276: 15369-15377.
  30. Angata K, Suzuki M, McAuliffe J, Ding Y, Hindsgaul O, Fukuda M. Differential biosynthesis of polysialic acid on neural cell adhesion molecule (NCAM) and oligosaccharide acceptors by three a2,8-sialyltransferases, ST8Sia IV (PST), ST8Sia II (STX), and ST8Sia III. J. Biol. Chem. 2000. 275: 18594-18601.
  31. Ogawa S, Yoshino R, Angata K, Iwamoto M, Pi M, Kuroe K, Matsuo K, Morio T, Urushihara H, Yanagisawa K, Tanaka Y. The mitochondrial DNA of Dictyostelium discoideum: complete sequence, gene content and genome organization. Mol. Gen. Genet. 2000. 263: 514-519.

English review

  1. Angata K, Fukuda M. Roles of polysialic acid in migration and differentiation of neural stem cells. Methods Enzymol. 2010. 479: 25-36.
  2. Angata K, Lee W, Mitoma J, Marth JD, Fukuda M. Cellular and molecular analysis of neural development of glycosyltransferase gene knockout mice. Methods Enzymol. 2006. 417: 25-37.
  3. Angata K, Fukuda M. Polysialyltransferases: major players in polysialic acid synthesis on the neural cell adhesion molecule. Biochimie 2003 85: 195-206.
  4. Nakayama J, Angata K, Ong E, Katsuyama T, Fukuda M. Polysialic acid, a unique glycan that is developmentally regulated by two polysialyltransferases, PST and STX in the central nervous system: from biosynthesis to function. Pathol. Int. 1998. 48: 665-677.

English books

  1. Narimatsu H, Suzuki Y, Aoki-Kinoshita K, Fujita N, Sawaki H, Shikanai T, Sato T, Togayachi A, Yoko-o T, Angata K, Kubota T, Noro E. Glycogene Database (GGDB) on the Semantic Web. In: A Practical Guide to Using Glycomics Databases. (Aoki-Kinoshita, K. ed.) 2017. Springer Japan, p163-175.
  2. Angata K, Fukuda M. ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3GAL1). In: Handbook of Glycosyltransferases and Related Genes (Taniguchi N, Honke K, Fukuda M, Narimatsu H, Yamaguchi Y, Angata T eds.) 2014. Springer-Verlag, p637-644.
  3. Angata K, Fukuda M. ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 (ST8SIA4). In: Handbook of Glycosyltransferases and Related Genes (Taniguchi N, Honke K, Fukuda M, Narimatsu H, Yamaguchi Y, Angata T eds.) 2014. Springer-Verlag, p805-812.
  4. Angata K, Sato T, Togayachi A, Narimatsu H. Beta1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2). In: Handbook of Glycosyltransferases and Related Genes (Taniguchi N, Honke K, Fukuda M, Narimatsu H, Yamaguchi Y, Angata T eds.) 2014 Springer-Verlag, p439-445.
  5. Angata K. Neurosphere culture and in vitro assay. 2012. GlycoPOD: http://jcggdb.jp/GlycoPOD/protocolShow.action?nodeId=t162
  6. Angata K, Fukuda M. Carbohydrates of neural cell glycoproteins: structure, biosynthesis and function. In: Neuroglycobiology (Fukuda M, Rutishauser U, Schnaar R, eds.) 2005. Oxford University Press, UK, p1-38.
  7. Nakayama J, Angata K, Suzuki M, Fukuda M. ST8Sia IV (PST-1). In: Handbook of Glycosyltransferases and Related Genes (Taniguchi N, Honke K, Fukuda M, eds.) 2002. Springer-Verlag, Tokyo, p340-346.

Japanese Books

  1. Tamiko Ono, Kiyohiko Agata Glycosmos Pathways, GDGDB, PACDB: Glycoforum 2020. Vol.23 (2), A5: Glycan and Database; https://glycoforum.gr.jp/article/23A5J.html
  2. Issaku Yamada, Kiyohiko Agata, Yu Watanabe, Tamiko Ono Database of glycoconjugates (GlyCosmos Glycoproteins and glycolipid, GlycoProtDB, GlycoNAVI-TCarp, GlycoPOST) Glycoforum 2020. Vol.23 (1), A2: Glycan and Database; https://glycoforum.gr.jp/article/23A2J.html
  3. Masae Hosoda, Kiyohiko Agata Current status of lectin databases (LfDB, LM-GlycomeAtlas, GlyCosmos Lectins - MCAW-DB) Glycoforum 2019. Vol.22 (4), A10: Glycan and Database; https://glycoforum.gr.jp/article/22A10J.html
  4. Akase, Sachiko, and Agata, Kiyohiko. Current status of databases of glycan-related genes (GGDB, FlyGlycoDB). Glycoforum 2019. Vol.22 (3), A7: Glycan and Database; https://www.glycoforum.gr.jp/article/22A7J.html
  5. Kiyohiko Agata and Atsushi Kuno, The role of glycans in dynamic homeostasis and its application to diagnostic techniques, Experimental Medicine, Disruption of homeostasis and diseases due to organ linkages (edited and supervised by Masato Kasuga), April 2019 special issue, 37 (7) p1031-1037.
  6. Kiyohiko Agata, Polysialic acid and neurogenesis, Pathology and Clinical Studies, Glycosylation and Disease (Jun Nakayama, ed.), Vol. 31, August 2013, p. 839-846
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