Takumi Kuwata | Faculty List | Soka University

Assistant Lecturer

Takumi Kuwata

Profile

Faculty of Science and Engineering Department of Life Science and Technology

Faculty of Science and Engineering Department of Science and Engineering for Sustainable Innovation

Specialized Field	Biophysics Bioinorganic Chemistry
Research theme	Structural and functional analysis of iron storage protein ferritin
research content	Iron is an essential element for maintaining life activities, but free Fe2+ causes cell damage through the Fenton reaction. For this reason, many organisms detoxify Fe2+ by oxidizing it to Fe3+ using a spherical, three-dimensional protein called ferritin, and store the oxidized iron as an insoluble iron mineral in the lumen of ferritin. However, the detailed molecular mechanism of the iron oxidation reaction by ferritin and the mechanism of iron storage are not clear. We are trying to elucidate the reaction mechanism by tracking these reactions using the stopped-flow method and performing kinetic analysis. By elucidating the molecular mechanism of these reactions related to iron metabolism, we are conducting research that aims to establish treatments for various diseases associated with abnormalities in iron metabolism in the future.
Subjects in charge	Earth Science Experiments Environmental Analytical Chemistry Experiment Physical Chemistry Experiments
Main career, work history, and academic background	2023 Soka University Graduate School Doctoral Program Completed Doctor of Science -------------------------------------- 2022 Japan Society for the Promotion of Science Research Fellow (DC2) 2023 Japan Society for the Promotion of Science Research Fellow (PD) 2024 Assistant Lecturer Soka University
Affiliated academic societies and organizations	Japanese Biochemical Society Protein Science Society of Japan Biophysical Society of Japan
Main Papers and Publications	Takumi Kuwata, Daisuke Sato, Yuki Yanagida, Eriko Aoki, Kazuo Fujiwara, Hideyuki Yoshimura, Masamichi Ikeguchi. “Morphological difference of Escherichia coli none-heme ferritin iron cores reconstituted in the presence and absence of inorganic phosphate.” Journal of Biological Inorganic Chemistry, vol. 27, pp.583-594, 2022. Takumi Kuwata, Yuta Okada, Tomoki Yamamoto, Daisuke Sato, Kazuo Fujiwara, Takuma Fukumura, Masamichi Ikeguchi, “Structure, Function, Folding and Aggregation of a Nueroferritinopathy-Related Ferritin Variant”, Biochemistry, vol. 58, no. 18, pp. 2318-2325, 2019.

Specialized Field	Biophysics Bioinorganic Chemistry
Research theme	Structural and functional analysis of iron storage protein ferritin
research content	Iron is an essential element for maintaining life activities, but free Fe2+ causes cell damage through the Fenton reaction. For this reason, many organisms detoxify Fe2+ by oxidizing it to Fe3+ using a spherical, three-dimensional protein called ferritin, and store the oxidized iron as an insoluble iron mineral in the lumen of ferritin. However, the detailed molecular mechanism of the iron oxidation reaction by ferritin and the mechanism of iron storage are not clear. We are trying to elucidate the reaction mechanism by tracking these reactions using the stopped-flow method and performing kinetic analysis. By elucidating the molecular mechanism of these reactions related to iron metabolism, we are conducting research that aims to establish treatments for various diseases associated with abnormalities in iron metabolism in the future.
Subjects in charge	Earth Science Experiments Environmental Analytical Chemistry Experiment Physical Chemistry Experiments
Main career, work history, and academic background	2023 Soka University Graduate School Doctoral Program Completed Doctor of Science -------------------------------------- 2022 Japan Society for the Promotion of Science Research Fellow (DC2) 2023 Japan Society for the Promotion of Science Research Fellow (PD) 2024 Assistant Lecturer Soka University
Affiliated academic societies and organizations	Japanese Biochemical Society Protein Science Society of Japan Biophysical Society of Japan
Main Papers and Publications	Takumi Kuwata, Daisuke Sato, Yuki Yanagida, Eriko Aoki, Kazuo Fujiwara, Hideyuki Yoshimura, Masamichi Ikeguchi. “Morphological difference of Escherichia coli none-heme ferritin iron cores reconstituted in the presence and absence of inorganic phosphate.” Journal of Biological Inorganic Chemistry, vol. 27, pp.583-594, 2022. Takumi Kuwata, Yuta Okada, Tomoki Yamamoto, Daisuke Sato, Kazuo Fujiwara, Takuma Fukumura, Masamichi Ikeguchi, “Structure, Function, Folding and Aggregation of a Nueroferritinopathy-Related Ferritin Variant”, Biochemistry, vol. 58, no. 18, pp. 2318-2325, 2019.

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