KUROSAWA Norio( Professor)


Specialized field Living organisms diversity/classification
Molecular biology
Applied microbiology
Classes Laboratory in Thesis Study 1
Exercise on Environmental Engineering for Symbiosis
Advanced Research 1
Thesis Study 1
Laboratory in Thesis Study 2
Advanced Research 2
Thesis Study 2
General Topics in Science and Engineering
Case Study 1
Case Study 2
Microbiology Laboratory
Practical Course in Oceanography
Microbial Ecology
Research theme 1. Taxonomy of thermophilic microorganisms
2. Taxonomy of cold adapted microorganisms
3. Development of host-vector system of thermoacidophilic archaea
4. Biogeography of zooplankton

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Detail of researcher information

Environmental Engineering for Symbiosis of Graduate school of engineering

  • Academic Experience EDUCATION:
    B.S. (1985) in Chemistry, Kitasato University (Japan)
    Ph.D. (1995) in Microbiology (taxonomy of thermoacidophilic archaea), Kitasato University (Japan)

    Qualitative and quantitative analysis of lipids from yeasts (1984, B.S. Thesis)
    Synthesis and analysis of liquid crystal compounds (1985-1990)
    Molecular analysis of memory and learning (1991-1995)
    Taxonomy and biochemistry of thermophiles (1991-the present, Ph. D. Thesis)
    Development of genetic manipulation system for thermophilic archaea. (1999-the present)
    Taxonomy and Biochemistry of Psychlophiles (2005-the present)
    Taxonomy of protistan microplankton (2006-the present)
    Biophylogeography of copepods (2012-present)
    Research Theme 1. Taxonomy, physiology, genetics, and molecular biology of theromophilic and Psychrophilic microorganisms.
    2. Taxonomy of protistan microplankton in Antactic lakes and Southern Ocean.
    3. Biophylogeography of copepods.
    Publications (2000-) S Suzuki and N Kurosawa. Disruption of the gene encoding restriction endonuclease SuaI and development of a host-vector system for the thermoacidophilic archaeon Sulfolobus acidocaldarius. Extremophiles. (in press)

    Satoh T, Yamamoto S, Kurosawa N. Chemical Characteristics of Solfataric-Acidic Pond Waters in the Kirishima Geothermal Area, Kagoshima Prefecture. Journal of Hot Spring Sciences. Sep;63(2):100-17 (2015)

    J Ueda, N Kurosawa. Characterization of an extracellular thermophilic chitinase from Paenibacillus thermoaerophilus strain TC22-2b isolated from compost. World Journal of Microbiology and Biotechnology 31 (1), 135-143 (2015)

    M Kosugi, N Kurosawa, A Kawamata, S Kudoh, S Imura. Year-round micrometeorological data from the habitats of terrestrial photosynthetic organisms in Langhovde, East Antarctica, during 2013. JARE data reports. Terrestrial biology 8, 1-6 (2015)

    SP Kok, K Tsuchiya, K Komatsu, T Toda, N Kurosawa. The protistan microplankton community along the Kuroshio Current revealed by 18S rRNA gene clone analysis: a case study of the differences in distribution interplay with ecological variability. Plankton and Benthos Research 9 (2), 71-82 (2014)

    J Ueda, S Yamamoto, N Kurosawa. Paenibacillus thermoaerophilus sp. nov., a moderately thermophilic bacterium isolated from compost. International journal of systematic and evolutionary microbiology 63 (Pt 9), 3330-3335 (2013)

    Satoh T, Watanabe K, Yamamoto H, Yamamoto S, Kurosawa N. Bacterial Community Structures in the Solfataric-Acidic Ponds in the Kirishima Geothermal Area, Kagoshima Prefecture. Journal of Hot Spring Sciences. Sep;63(2):100-17 (2013)

    M Nishiyama, S Yamamoto, N Kurosawa. Microbial community analysis of a coastal hot spring in Kagoshima, Japan, using molecular-and culture-based approaches. Journal of Microbiology 51 (4), 413-422 (2013)

    T Satoh, K Watanabe, H Yamamoto, S Yamamoto, N Kurosawa. Archaeal community structures in the solfataric acidic hot springs with different temperatures and elemental compositions. Archaea 2013 (2013)

    N Kurosawa. Discovery of Thermostable Enzymes from Hot Environmental Samples by Metagenomic Approaches (Chapter 16). In Satyanarayana T et al (eds) Thermophilic Microbes in Environmental and Industrial Biotechnology, 2nd ed.: Biotechnology of Thermophiles. Springer Science+Business Media, Dordrecht. 2013 Apr:413-427

    Bin Haji Mohd Taha AI, Ahmed RZ, Motoigi T, Watanabe K, Kurosawa N, Okuyama H. Lipids in Cold-adapted Microorganisms (Chapter 11). In Yumoto I (ed) Cold-adapted Microorganisms. Caister Academic Press, Norfolk. 2013 Aug:189-214.

    SP Kok, T Kikuchi, T Toda, N Kurosawa. Diversity analysis of protistan microplankton in Sagami Bay by 18S rRNA gene clone analysis using newly designed PCR primers. Journal of oceanography 68 (5), 599-613 (2012)

    Kok SP, Kikuchi T, Toda T, Kurosawa N. (2012). Diversity and community dynamics of protistan microplankton in Sagami Bay revealed by 18S rRNA gene clone analysis. Plankton Benthos Res Vol.7, p.75–86.

    N Nagao, N Tajima, M Kawai, C Niwa, N Kurosawa, T Matsuyama, FM Yusoff, T Toda. Maximum organic loading rate for the single-stage wet anaerobic digestion of food waste. Bioresource technology 118, 210-218 (2012)

    Kawasaki Y, Aoki M, Makino Y, Sakai H, Tsuboi Y, Ueda J, Sonoda K, Watanabe K, Yamamoto S, Kurosawa N. (2011). Characterization of moderately thermophilic bacteria isolated from saline hot spring in Japan. Microbiology Indonesia, Vol.5, p.56-60.

    Kimata-Kino, N., Ikeda, S., Kurosawa, N. & Toda, T. (2011). Saline adaptation of granules in mesophilic UASB reactors. International Biodeterioration & Biodegradation 65, 65-72.

    Kurosawa N, Sato S, Kawarabayasi Y, Imura S, Naganuma T. (2010). Archaeal and bacterial community structures in the anoxic sediment of Antarctic meromictic lake Nurume-Ike. Polar Science, Vol.4, p.421-429.

    Watanabe, K., Nagao, N., Toda, T. & Kurosawa, N. (2010). Bacterial community in the personal-use composting reactor revealed by isolation and cultivation-independent method. Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Wastes 45, 372-378.

    Watanabe, K., Nagao, N., Toda, T. & Kurosawa, N. (2009). The dominant bacteria shifted from the order "Lactobacillales" to Bacillales and Actinomycetales during a start-up period of large-scale, completely-mixed composting reactor using plastic bottle flakes as bulking agent. World Journal of Microbiology & Biotechnology 25, 803-811.

    Miyaguchi, H., Kurosawa, N. & Toda, T. (2008). Real-time polymerase chain reaction assays for rapid detection and quantification of Noctiluca scintillans zoospore. Marine Biotechnology 10, 133-140.

    Nagao, N., Watanabe, K., Osa, S., Matsuyama, T., Kurosawa, N. & Toda, T. (2008). Bacterial community and decomposition rate in long term fed-batch composting using woodchip and Polyethylene terephthalate (PET) as bulking agents. World Journal of Microbiology & Biotechnology 24, 1417-1424.

    Watanabe, K., Nagao, N., Toda, T. & Kurosawa, N. (2008). Changes in bacterial communities accompanied by aggregation in a fed-batch composting reactor. Current Microbiology 56, 458-467.

    Watanabe, K., Nagao, N., Yamamoto, S., Toda, T. & Kurosawa, N. (2007). Thermobacillus composti sp nov., a moderately thermophilic bacterium isolated from a composting reactor. International Journal of Systematic and Evolutionary Microbiology 57, 1473-1477.

    Kurosawa N, Grogan DW. Homologous recombination of exogenous DNA with the Sulfolobus acidocaldarius genome: properties and uses. FEMS Microbiol. Lett. Vol.253, p.141-149 (2005)

    Watanabe K, Nagao N, Toda T, Kurosawa N. Bacterial communities in solid-state fermentation reactor for organic waste composting. Proceedings of 7th World Congress of Chemical Engineering (Manuscripts CD-ROM), Glasgow, July (2005)

    Kurosawa N, Itoh YH, Itoh T. T. kawarayensis sp. nov., a new member of the genus Thermus, isolated from Japanese hot springs. Extremophiles, Vol.8, p.1433-4909 (2004)

    Kurosawa N, Itoh YH, Itoh T. Sulfolobus hakonensis Takayanagi et al. 1996 as Metallosphaera hakonensis comb. nov. based on phylogenetic evidence and DNA G+C content. Int. J. Syst. Evol. Microbiol., Vol.57, p.1607-1608 (2003)

    Cosper NJ, Eby DM, Kounosu A, Kurosawa N, Neidle EL, Kurtz DM Jr, Iwasaki T, and Scott RA. Redox-dependent structural changes in archaeal and bacterial Rieske-type [2Fe-2S] clusters. Protein Sci., Vol.11, p.2969-2973 (2002)

    Itoh YH, Kurosawa N, Uda I, Sugai A, Tanoue S, Itoh T, Horiuchi T, and Itoh T. Metallosphaera sedula TA-2, a calditoglycerocaldarchaeol deletion strain of a thermoacidophilic archaeon. Extremophiles, Vol.5, p.241-245 (2001)

    Iwai T, Kurosawa N, Itoh YH, and Horiuchi T. Phylogenetic analysis of archaeal PCNA homologues. Extremophiles, Vol.4, p.357-364 (2000)

    Iwai T, Kurosawa N, Itoh YH, Kimura N, and Horiuchi T. Sequence analysis of three family B DNA polymerases from the thermoacidophilic crenarchaeon Sulfurisphaera ohwakuensis. DNA Res., Vol.7, p.243-251 (2000)

    Kurosawa N, Fukuda K, Itoh YH, and Horiuchi T. Partial purification and characterization of thermostable acid phosphatase from thermoacidophilic archaeon Sulfolobus acidocaldarius. Current Microbiol., Vol.40, p57-60 (2000)